Characterization of Nora Virus Structural Proteins via Western Blot Analysis

Figure 1

Two distinct sized particles are produced from a Nora virus infection of Drosophila melanogaster witi Rel^E23 strain flies. Approximately 3,000 Nora virus infected flies were homogenized and processed as described in Section 2. In (a) a representative CsCl gradient is shown, depicting the two bands of virus particles obtained. The bands were collected individually and dialyzed against 10 mM Tris-HCl, pH 8.0, and 250 mM NaCl buffer. Electron micrographs of the particles obtained from the upper band (b) and the lower band (c) are shown. The average sizes of the virus particles were 31.8 nm (upper band; ) and 29.7 nm (lower band; ), respectively.