Chlorophyllin Bait Formulation and Exposure to Different Spectrum of Visible Light on the Reproduction of Infected/Uninfected Snail Lymnaea acuminata

Table 3

Effect of sublethal doses (40% and 80% of 24 h LC₅₀) of chlorophyllin bait containing serine and starch (in red light/sunlight) on the fecundity, hatchability, and survival of the infected snails L. acuminata eggs.

Exposure	LC50 24 h	Sublethal dose (mg/L)	Fecundity %				Hatchability %	Survivability %
Uninfected snails			24 h	48 h	72 h	96 h		24 h	48 h	72 h
Control	—	—	220 ± 0.15	180 ± 1.2	150 ± 1.3	100 ± 2.0	95.2 ± 2.0 (7–9)	96 ± 0.3	89 ± 3.2	50 ± 0.3
Red light starch	7.05	40% (2.82)	122.5 ± 0.84	67.5 ± 0.48	0	0	9.25 ± 0.5 (9–11)	88 ± 0.5	36.4 ± 0.2	0
		80% (5.64)	106 ± 0.81	37.5 ± 0.48	0	0	0	0	0	0
Red light serine	7.89	40% (3.15)	99.5 ± 0.04	11.2 ± 0.28	0	0	19.25 ± 0.8 (15–17)	37.4 ± 0.6	47.1 ± 0.5	0
		80% (6.31)	80.25 ± 0.16	18.5 ± 0.10	0	0	0	0	0	0
Sunlight starch	9.89	40% (3.94)	135 ± 0.96	32.25 ± 0.07	0	0	23.5 ± 0.92 (10–12)	42.6 ± 0.20	37.4 ± 0.5	0
		80% (7.88)	108 ± 0.28	29.5 ± 0.19	0	0	0	0	0	0
Sunlight serine	10.93	40% (4.37)	67 ± 0.93	32.5 ± 0.45	0	0	28.5 ± 0.09 (12-13)	42.6 ± 0.2	36.4 ± 0.5	0
		80% (8.74)	55.25 ± 1.0	14.25 ± 0.8	0	0	21.5 ± 0.10 (15–19)	43 ± 0.4	31 ± 0.1	0

Each experiment was repeated six times and the values of fecundity, hatchability, and survival were the mean of six replicates. Significant () difference was noted when Student’s -test was applied to treated and control groups.
⁺Product moment correlation coefficient showed that there was significant () negative correlation in between exposure period and fecundity of L. acuminata snail. (—) shows no dose; (0) shows no fecundity, hatchability, and survivability.