Virulence and Antimicrobial Resistance in Campylobacter spp. from a Peruvian Pediatric Cohort

Table 1

Primers and PCR conditions used in the present study.

Target	Description	Primer (5′-3′)	Size (bp)	Ann	Cycles	Ref
Identification
C. coli		AGGCAAGGGAGCCTTTAATC	364	61	30	[23]
		TATCCCTAT CTACAAATTCGC
C. jejuni		CATCTTCCCTAGTCAAGCCT	773	61	30	[23]
		AAG ATATGGCACTAGCAAGAC
Resistance
gyrA	DNA-Gyrase subunit A	ATGATGAGGCAAAAAGAGA	410	55	30	[8]
		TAAACTATGAGGTGGGATGT
23S rRNA		GTAAACGGCGGCCGTAACTA	699	52	35	[24]
		GACCGAACTGTCTCACGACG
Virulence
cadF	Campylobacter adhesin to fibronectin	TTGAAGGTAATTTAGATATG	400	45	30	[23]
		CTAATACCTAAAGTTGAAAC
cdtABC	Cytolethal distending Toxin subunits ABC	GGAAATTGGATTTGGGGCTATACT	1215	55	30	[23]
		TTGCACATAACCAAAAGGAAG
cdtA	Cytolethal distending Toxin subunit A	CCTTGTGATGCAAGCAATC	370	42	30	[23]
		ACACTCCATTTGCTTTCTG
cdtB	Cytolethal distending Toxin subunit B	GTTAAAATCCCCTGCTATCAACCA	495	42	30	[23]
		GTTGGCACTTGGAATTTGCAAGGC
cdtC	Cytolethal distending Toxin subunit C	CGATGAGTTAAAACAAAAAGATA	182	42	30	[23]
		TTGGCATTATAGAAAATACAGTT
iam1	Invasión-associated marker 1	GCGCAAAATATTATCACCC	518	52	30	[23]
		TTCACGACTACTATGCGG
iam2	Invasion-associated marker 2	GGCGCTTTAGGGAAGCTG	1360	52	30	[23]
		CTTTAAATTGAATCACGGG
iam3	Invasion-associated marker 3	TGAGGAGCTAAGGGTGCAAA	270	52	30	[23]
		AATACTGATATTTTCCACAT

bp: base pair; Ann: annealing; Ref: reference. Primers used in a Multiplex PCR.