Induction of Reactive Intermediates and Autophagy-Related Proteins upon Infection of Macrophages with Rhodococcus equi

Figure 2

R. equi infection of BMDM led to an increase in ROS. BMDM were uninfected or infected with R. equi (moi3) for 1 h, 3 h, 6 h, 18 h, 24 h, 48 h, and 72 h. Stimulation of BMDM with a combination of 10 μg/ml LPS and 25 ng/ml IFNγ (24 h) is used as a positive control. R. equi infection led to an increase in ROS as measured using a ROS indicator, CM-H2DCFDA (30 min). Data represent mean ± SEM of 6 independent measurements. .