Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase from Bacillus megaterium Using Response Surface Methodology
The effect of temperature on glucose isomerase production. Enzyme activity was increased along with an elevation of temperature from 33°C to 37°C (). When the temperature increased over optimal temperature (39°C–43°C), the enzyme activity dramatically decreased from 0.238 ± 0.001 to 0.206 ± 0.020 U/mg biomass (). From these data, we chose 37°C as the optimal temperature for glucose isomerase production. a, b, c, d, and e indicate the significant difference between groups.