Assessing the Effect of SNPs on Litter Traits in Pigs

Table 2

Oligonucleotide primers and restriction endonucleases to identify the SNPs under study.

Number of SNPs	SNP	Oligonucleotide primers	Annealing temperature	Length of amplicon (bp)	Restriction endonuclease/PSQ	Length of restriction fragments (bp)
SNP_1	rs80956812	F5′-GGCGCTAAGTGAGCTCTTG-3′; R5′-CCCTCACTGATGCAACTCTAAA-3′		296	Bme18 I	202, 94
SNP_2	rs81471381	F5′-CCACGCTCTCTACAAGCCAA-3′; R5′-CCCCATTCACGGTCTTGGAA-3′		540	SfaN I	368, 172
SNP_3	rs80891106	F5′-TGACAAGCTTCAGACAGTTCCT-3′; R5′-TGCACTGAACCTTCACACACA-3′		332	PSQ
SNP_4	rs81399474	F5′-AGAACGAGGCTTCTTCCTGTT-3′; R5′-ACAGTCTAAAGCCTGATTTCCCT-3′		297	PSQ
SNP_5	rs81421148	F5′-TTCTGTACTTCTCCATCACAAGAA-3′; R5′-CGAAGACTTGTTTACGCATCATAG-3′		383	Mnl I	276, 107
SNP_6	rs81242222	F5′-TGCAGAGATTCCAGCAAGCC-3′; R5′-CATCTGGTTGGTTTGGTCGTG-3′		290	PSQ
SNP_7	rs81319839	F5′-GAAGCACCCAATGGGACTCT-3′; R5′-ATGAGGTTGTCTTGGCACCAT-3′		356	BstMB I	276, 80
SNP_8	rs81312912	F5′-ACAGGACAGTATGAAAAATCTGTTG-3′; R5′-GCTTCCCCCAGAAAGGACTG-3′		302	BstAU I	241, 61
SNP_9	rs80962240	F5′-ATGGAGGAACCGGCTATGTG-3′; R5′-GCAGTCCTGCCCATGAGTAT-3′		461	Vsp I	350, 111