Histological Analysis of Bone Callus in Delayed Union Model Fracture Healing Stimulated with Pulsed Electromagnetic Fields (PEMF)

Figure 2

Microscopic images of longitudinal sections of the femur were stained with safranin O fast green (SOFG) on day 10. Bone callus consisted of fibrous, cartilage, and bone tissue. Using SOFG staining, cartilage tissue is observed as reddish-orange, while other connective tissues are observed as green. Longitudinal sections of bone at 40x magnification and several sections of bone at 200x magnification were observed (yellow square line).