Synthesis and Diuretic Activity of Substituted 1,3,4-Thiadiazoles

Table 2

The effect of the compounds on 24 hours of urine volume of mice.

Group	Volume of urine (ml)
	1 hr	2 hr	3 hr	4 hr	5 hr	24 hr
Cont.	0.02 ± 0.01	0.13 ± 0.06	0.33 ± 0.04	0.37 ± 0.03	0.67 ± 0.06	1.13 ± 0.67
Fr10	1.12 ± 0.14^a3c3d3e3f3g3h3i3	1.48 ± 0.14^a3c3d3e3f3g3h3i3	1.63 ± 0.14^a3c3d3e3f3g3h3i3	1.73 ± 0.14^a3c3d3e3f3g3h3i3	1.90 ± 0.17^a3c2d2e3f2g3h3i3	2.77 ± 0.14^a3c2d2e3h3i3
CPD-І	0.37 ± 0.12	0.75 ± 0.09^a2e1	0.88 ± 0.16^a2e1	0.97 ± 0.04^a3e2	1.27 ± 0.06^a2e2	2.02 ± 0.10^a3h1
CPD-II	0.33 ± 0.06	0.48 ± 0.12	0.88 ± 0.06^a2e1	1.0 ± 0.06^a3e2	1.23 ± 0.08^a2e2	1.93 ± 0.08^a2
CPD-III	0.08 ± 0.04	0.20 ± 0.08	0.27 ± 0.09	0.47 ± 0.07	0.57 ± 0.09	1.50 ± 0.03
CPD-IV	0.18 ± 0.04	0.40 ± 0.05	0.97 ± 0.07^a3e3	1.12 ± 0.05^a3e3	1.32 ± 0.09^a2e1	2.35 ± 0.11^a3e2h3i1
CPD-V	0.03 ± 0.03	0.30 ± 0.05	0.78 ± 0.05^a2e2	0.95 ± 0.07^a3e2	1.13 ± 0.13^e2	2.13 ± 0.12^a3e1h2
CPD-VI	0.27 ± 0.09	0.55 ± 0.12	0.72 ± 0.07^a3e2	0.82 ± 0.09^a1	0.87 ± 0.09	1.42 ± 0.11
CPD-VII	0.30 ± 0.01	0.63 ± 0.16^a1	0.85 ± 0.10^a2e1	0.90 ± 0.13^a2e1	1.03 ± 0.10	1.75 ± 0.26^a1

Values are expressed as mean ± S.E.M (n = 6); analysis was performed with one-way ANOVA followed by Tukey test; ^aagainst control, ^bagainst standard drug, ^cagainst CPD-І, ^dagainst CPD-II, ^eagainst CPD-III; ^fagainst CPD-IV; ^gagainst CPD-V; ^hagainst CPD-VI; ⁱagainst CPD-VII;¹, ², ³; cont: 2% Tween-80 in water; Fr10: furosemide 10 mg/kg; CPD-I: S-5-methyl-1, 3, 4-thiadiazol-2-yl 4-chlorobenzothioate; CPD-II: S-5-methyl-1, 3, 4-thiadiazol-2-yl 4-methoxybenzothioate; CPD-III: S-5-amino-1, 3, 4-thiadiazol-2-yl 4-nitrobenzothioate; CPD-IV: S-5-methyl-1, 3, 4-thiadiazol-2-yl 4-nitrobenzothioate; CPD-V: S-5-methyl-1, 3, 4-thiadiazol-2-yl 4-chlorobenzothioate; CPD-VI: S-5-amino-1, 3, 4-thiadiazol-2-yl propanethioate; CPD-VII: S-5-amino-1, 3, 4-thiadiazol-2-yl 4-methoxybenzothioate.