Synthesis and Diuretic Activity of Substituted 1,3,4-Thiadiazoles

Table 4

The effect of the compounds on 24 hours of urine electrolyte excretion of mice.

Group	Urinary electrolyte excretion (mmol/L)			Saluretic index			Na^+/K^+	Cl^−/Na^++K^+
	Na^+	K^+	Cl^−	Na^+	K^+	Cl^−
Cont.	51.17 ± 4.61	56.50 ± 6.20	47.91 ± 5.63				0.9	0.44
Fr10	100.67 ± 5.00^a3e1	116.26 ± 16.54^a3d1e1i1	128.03 ± 5.37^a3c3d3e3f3g3h3i3	1.97	2.06	2.67	0.87	0.59
CPD-І	84.50 ± 8.20^a1	74.56 ± 8.32	73.95 ± 3.55^a1	1.65	1.32	1.54	1.13	0.46
CPD-II	89.67 ± 8.04^a2	102.69 ± 12.73^a1	76.38 ± 4.46^a2	1.75	1.82	1.59	0.87	0.40
CPD-III	78.17 ± 6.38	72.05 ± 6.42	66.98 ± 4.94	1.53	1.28	1.39	1.08	0.45
CPD-IV	97.83 ± 3.35^a3	99.80 ± 6.11^a1	75.38 ± 5.97^a2	1.91	1.77	1.57	0.98	0.38
CPD-V	83.83 ± 8.83^a1	102.66 ± 8.95^a1	79.15 ± 4.85^a2	1.67	1.82	1.65	0.82	0.42
CPD-VI	70.50 ± 5.87	67.18 ± 4.98	61.91 ± 4.68	1.37	1.19	1.29	1.04	0.45
CPD-VII	78.33 ± 6.58	70.60 ± 4.44	65.11 ± 3.77	1.53	1.25	1.36	1.11	0.44

Values are expressed as mean ± S.E.M (n = 6); analysis was performed with one-way ANOVA followed by Tukey test; ^aagainst control, ^bagainst standard drug, ^cagainst CPD-І, ^dagainst CPD-II, ^eagainst CPD-III; ^fagainst CPD-IV; ^gagainst CPD-V; ^hagainst CPD-VI; ⁱagainst CPD-VII;¹, ², ³; cont: 2% Tween-80 in water; Fr10: furosemide 10 mg/kg; CPD-I: S-5-methyl-1, 3, 4-thiadiazol-2-yl 4-chlorobenzothioate; CPD-II: S-5-methyl-1, 3, 4-thiadiazol-2-yl 4-methoxybenzothioate; CPD-III: S-5-amino-1, 3, 4-thiadiazol-2-yl 4-nitrobenzothioate; CPD-IV: S-5-methyl-1, 3, 4-thiadiazol-2-yl 4-nitrobenzothioate; CPD-V: S-5-methyl-1, 3, 4-thiadiazol-2-yl 4-chlorobenzothioate; CPD-VI: S-5-amino-1, 3, 4-thiadiazol-2-yl propanethioate; CPD-VII: S-5-amino-1, 3, 4-thiadiazol-2-yl 4-methoxybenzothioate.