Optimization and Validation of ELISA for Aflatoxin B1 Detection in Fermented Forages and Feeds

Table 4

Repeatability of the ELISA method for corn silage. Two operators measured AFL B1 in three repetitions on three different days from freshly prepared samples. SD, standard deviation. Spike: 35 ppb AFL B1.

Corn silage	Repetition	1st day AFL B1 (ppb)	2nd day AFL B1 (ppb)	3rd day AFL B1 (ppb)
Daily	Mean	29.17	27.04	28.11
	SD	4.30	3.23	1.95
Significance test between days results	T	t crit (95%)	Result	Evaluation
1-2	0.9669	2.2621	t < tcrit	No significant difference
2-3	−0.6907	2.3060	t < tcrit	No significant difference
1–3	0.5501	2.3646	t < tcrit	No significant difference
Significance test between operators	t	t crit (95%)	Result	Evaluation
Operator 1-operator 2	−0.1734	2.2281	t < tcrit	No significant difference