In Vitro Regeneration of Korarima (Aframomum corrorima (Braun) P. C. M. Jansen): A Threatened Spice and Medicinal Herb from Ethiopia

Figure 2

In vitro regeneration of korarima: (a) rhizomes cultured on callus induction media; (b) friable callus produced on the MS medium containing 3.0 mg/l 2,4-D combined with 0.5 mg/l KN after 10 weeks; (c) friable callus ready for shoot regeneration; (d) shoots developed from callus on shoot induction MS medium containing 2.0 mg/l TDZ combined with 0.5 mg/l IBA after two weeks; (e) shoots regenerated on 2.0 mg/l TDZ combined with 0.5 mg/l IBA after four weeks; (f) shoots proliferated after the transfer of shoot tips on 2.0 mg/l TDZ combined with 0.5 mg/l IBA; (g) shoots proliferated after the transfer of shoot tips on 2.0 mg/l BAP combined with 0.5 mg/l IBA. Bars = 1 cm.

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