The toxicity of histamine has attracted numerous researchers to develop a method for histamine determination purposes. The Food and Drug Administration (FDA) unequivocally prohibits the consumption of histamine above 50 mg·kg<sup>−1</sup>. Thus, an innovation in histamine detection in fish has been developed in this research. The investigation of the histamine level in fish has been conducted by using an electrochemical sensor approach and producing a polymer via molecularly imprinted polymer (MIP) on a screen-printed electrode. The technique was validated by assessing the shifts in electron shifting using the cyclic voltammetry (CV) approach and electrochemical impedance spectroscopy (EIS), whereas differential pulse voltammetry (DPV) was applied to validate the sensor method. The instruments showed a linear response ranging from 1–1000 nmol·L<sup>−1</sup>, with a detection limit of MIP/SPE at 1.765 nmol·L<sup>−1</sup> and 709 nmol·L<sup>−1</sup> for the NIP/SPE, respectively. The sensing technique was employed to determine the histamine level in selected samples at room temperature (25°C). The outcomes of this study indicated that the validated chemical sensor allowed accurate and precise detection of fish samples and can be categorized as a simple approach. The instrument is inexpensive and suitable for on-site detection.

Muhammad Abdurrahman Munir

Jamia Azdina Jamal

Mazlina Mohd Said

Sofian Ibrahim

Mohamad Syahrizal Ahmad

Polyurethane Application to Transform Screen-Printed Electrode for Rapid Identification of Histamine Isolated from Fish

The production of nonindustrial cannabis is highly developed in the Moroccan Rif region; however, local farmers consider hemp seeds which are rich in omega 3 and tocopherols, only as by-products of cannabis cultivation with low market value. The local ecotype is considered to be a plant with a cannabinoid content of more than 0.4%. So, the objective of this research is to investigate how the incorporation of this local hemp seed affects productive performance and egg quality traits. The experiment is conducted to evaluate the effects of hemp seed (HS) incorporation on hen laying performance and physical egg quality at three levels: 10% (HS-10% group), 20% (HS-20% group), and 30% (HS-30% group). Ninety-six Lohmann Brown classic laying hens were randomly assigned to a control group and three feed treatments. The sampling was taken after the 28-week rearing period (peak egg laying). Throughout the experiment, low-rate HS inclusion (HS-10%) showed no significant differences in egg-laying performance (<svg xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg" style="vertical-align:-3.42943pt" id="M1" height="10.2124pt" version="1.1" viewBox="-0.0498162 -6.78297 7.83752 10.2124" width="7.83752pt"><g transform="matrix(.013,0,0,-0.013,0,0)"><path id="g113-113" d="M570 304C570 398 525 448 414 448C385 448 343 445 312 434L329 511L321 518C297 504 262 482 244 460L233 411C195 397 159 381 128 358L135 332C160 347 189 360 224 373L111 -147C97 -210 84 -218 17 -231L13 -257L254 -247L259 -218L233 -216C183 -212 177 -202 189 -142L218 -1C238 -10 266 -12 283 -12C351 3 429 48 483 105C543 168 570 242 570 304ZM482 289C482 161 380 33 304 33C278 33 248 51 233 69L303 396C326 400 352 403 369 403C428 403 482 380 482 289Z"/></g></svg> > 0.05). However, the high incorporation rates of HS (20% and 30%) negatively affected the egg-laying performance (84–94% and 80–86%, respectively). The albumen quality was also improved by the HS inclusion, where the highest values of the Haugh unit were recorded, ranging between 68.69 and 73.91 for the HS-30% groups. The results also show that HS inclusion and duration influence significantly the yolk color (<svg xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg" style="vertical-align:-3.42943pt" id="M2" height="10.2124pt" version="1.1" viewBox="-0.0498162 -6.78297 7.83752 10.2124" width="7.83752pt"><g transform="matrix(.013,0,0,-0.013,0,0)"><use xlink:href="#g113-113"/></g></svg> < 0.001). The yellow intensity decreases with HS incorporation and aging, from a dark yellow (<svg xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg" style="vertical-align:-0.2063999pt" id="M3" height="10.3089pt" version="1.1" viewBox="-0.0498162 -10.1025 12.2985 10.3089" width="12.2985pt"><g transform="matrix(.013,0,0,-0.013,0,0)"><path id="g113-99" d="M452 333C452 398 425 448 375 448C329 448 235 404 140 292H138L229 683C233 701 234 712 225 712C208 712 149 686 66 677L64 652H97C135 652 140 651 129 598L38 167C23 96 29 57 44 33C60 7 98 -12 132 -12C169 -12 232 3 290 41C383 102 452 223 452 333ZM365 316C365 199 308 87 239 49C221 39 200 35 183 35C137 35 99 70 112 163C116 191 123 215 129 233C190 316 290 392 330 392C348 392 365 372 365 316Z"/></g><g transform="matrix(.0091,0,0,-0.0091,6.101,-5.741)"><path id="g50-43" d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 158Z"/></g></svg> = 38.63 for the control group) to a very pale yellow (<svg xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg" style="vertical-align:-0.2063999pt" id="M4" height="10.3089pt" version="1.1" viewBox="-0.0498162 -10.1025 12.2985 10.3089" width="12.2985pt"><g transform="matrix(.013,0,0,-0.013,0,0)"><use xlink:href="#g113-99"/></g><g transform="matrix(.0091,0,0,-0.0091,6.101,-5.741)"><use xlink:href="#g50-43"/></g></svg> = 26.29 for HS 30% group). Based on these findings, we can conclude that the incorporation of nonindustrial Moroccan cannabis seeds (ecotype <i>Beldiya</i>) at low rate in the diet of laying hens does not alter the laying performance or the quality of the egg; therefore, they could be used in poultry feeding as an alternative constituent to partially replace high-cost imported ingredients, such as corn and soybeans.

Yassine Taaifi

Kamal Belhaj

Farid Mansouri

Youssef Rbah

Najlae Elbouanani

Reda Melhaoui

Abdesamad Ben Moumen

Embarek Azeroual

Hana Serghini-Caid

Ahmed Elamrani

Impact of Cannabis Seed Incorporation in Layer Diet on Productive Performance and Egg Quality Traits

Karina Karina

Louis Martin Christoffel

Rita Novariani

Imam Rosadi

Iis Rosliana

Siti Rosidah

Siti Sobariah

Novy Fatkhurohman

Nurlaela Puspitaningrum

Yuli Hertati

Irsyah Afini

Difky Ernanda

Tias Widyastuti

A. D. Sulaeha

Alfida Zakiyah

Noor Aini

Grady Krisandi

Hubert Andrew

Corrigendum to “The Effect of Intravenous Autologous Activated Platelet-Rich Plasma Therapy on “Profibrotic Cytokine” IL-1<i>β</i> Levels in Severe and Critical COVID-19 Patients: A Preliminary Study”

The influence of vermicompost and various concentrations of lead on the activity of hydrolytic (urease and protease) and oxidative-reducing (catalase and dehydrogenase) enzymes in the sierozem soil of Southern Kazakhstan (Turkestan region) was studied. Background unpolluted soils served as a control. The work shows a change in the enzymatic potential when vermicompost (8 t/ha) and lead are introduced into the soil in the concentration range of 16 to 160 mg/kg Pb. As a result of experimental studies, a decrease in the activities of catalase, protease, and dehydrogenase and, conversely, an increase in the activity of urease with an increase in the lead content in the soil system were revealed. Introduction of vermicompost (vermicompost) into the soil caused an increase in the activity of all studied enzymes and a decrease in the translocation ability of Pb. Inhibition of the process of translocation of lead into plants by vermicompost creates conditions for obtaining environmentally friendly agricultural products.

G. A. Sainova

N. A. Kaliyeva

D. Kh Yuldashbek

A. D. Akbasova

The Influence of Vermicompost and Various Concentrations of Lead on the Enzymatic Activity of Sierozem Soils of Kazakhstan

Through the electrodeposition technique, toxic metals in wastewater can be removed and deposited on a chosen substrate with excellent selectivity. In this work, we use this technique to extract lead cations from simulated wastewater by using fluorine-doped tin oxide (FTO) substrate at various temperatures. In situ tracking of lead nucleation at advanced stages has been achieved by chronoamperometry. According to the experimental results, the theoretical models developed to study the kinetic growth of lead deposits in 2D and 3D are in good agreement. Nucleation rate and growth rate constants, for example, were found to be strongly influenced by temperature. Cottrell’s equation is used to calculate the diffusion coefficient. X-ray diffraction, scanning electron microscopy, and energy-dispersiveX-ray techniques were used to investigate and characterize the lead deposits. The reported results could provide insight into the optimization of electrodeposition processes for heavy metal recovery from wastewater and electronic wastes.

Ridha Hamdi

Amani Rached

Amal L. Al–Otaibi

Imen Massoudi

Shouq Alkorbi

Amor Saidi Ben Ali

Physical, Static, and Kinetic Analysis of the Electrochemical Deposition Process for the Recovery of Heavy Metal from Industrial Wastewater

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Scientifica publishes research articles and review articles covering a wide range of subjects in the life sciences, environmental sciences, health sciences, and medicine. 

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