Research Article

Sleep Recovery Restored Neuroglobin Immunoreactivity in Rat LDTg-PPTg Nuclei

Figure 2

The sleep-restored neuroglobin (Ngb) immunoreactivity. (a) The total number of Ngb-positive cells after three and six hours of sleep recovery. Sleep deprivation (SD) reduces the Ngb-positive cells in all nuclei analyzed compared to control (Ctrl) ((a) vs. (b)). No significant difference was observed between the Ctrl group versus three and six hours of sleep recovery. Significant differences were found between sleep recovery groups vs. SD. Pedunculopontine tegmentum (PPTg) and laterodorsal tegmentum (LDTg) nuclei. The next panel is representative photomicrographs of LDTg sections from Ctrl (b), SD (c), and SD + 3 h of sleep recovery (d) groups, respectively. Arrow indicates Ngb-positive cells. Data are expressed as the meanSEM. Magnification 20x. The rectangle (μm) represents the area that was counted. Scale bar: 50 μm. .
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