Stroke Status Evoked Adhesion Molecule Genetic Alterations in Astrocytes Isolated from Stroke-Prone Spontaneously Hypertensive Rats and the Apigenin Inhibition of Their Expression

Figure 8

The inhibitory effects of apigenin and pioglitazone on U937 cell adhesion in astrocytes isolated from SHRSP. The astrocytes isolated from SHRSP/IZM were cultured for 24 hours with or without 10, 30, or 50 $\mu$M of apigenin or pioglitazone and were then challenged with TNF-$\alpha$ (10 ng/mL). The U937 cells (1 $\times$ 10 ⁶ cells/mL) were added and incubated with astrocytes at ${37}^{°}\text{C}$ for 3 hours. The adhesion of U937 cells to the astrocytes was measured using quantitative monolayer adhesion assays, similar to those previously described [28]. The inhibitory effects of apigenin are indicated (fold). The number of bound U937 cells per square millimeter was determined by direct microscopy. The data are means ± S.D (n = 6).