Lentiviral-Mediated shRNA Silencing of PDE4D Gene Inhibits Platelet-Derived Growth Factor-Induced Proliferation and Migration of Rat Aortic Smooth Muscle Cells
Figure 3
Knockdown of endogenous PDE4D inhibits PDGF-induced VSMC migration. PDGF-induced VSMC migration was assessed in a Boyden’s chamber. Representative images (a, b, c) and group data (d) are shown. (a)–(c) Representative photomicrographs illustrating migration of VSMCs. (a) Migration of VSMCs stimulated by PDGF-BB. (b) Migration of VSMCs stimulated by PDGF-BB in cells infected with lentivirus particles carrying nonsense shRNA. (c) Migration of VSMCs stimulated by PDGF-BB in cells infected with lentivrius carrying shRNA against PDE4D. (d) Bar graph illustrating PDGF-BB-induced VSMC migration in noninfected cells and cells infected with lentivirus particles carrying either PDE4D or nonsense shRNA. Numbers of migrated VSMCs were much less in cells infected with lentivrius carrying PDE4D shRNA compared with noninfected cells or cells infected with lentivirus particles carrying nonsense shRNA. Bars are the mean plus minus SEM of six random fields of view from three separate experiments. Original magnification, ×400. *P less than .05 versus control. #P less than .05 versus nonsense shRNA.
