Cold Atmospheric Plasma Inhibits the Proliferation of CAL-62 Cells through the ROS-Mediated PI3K/Akt/mTOR Signaling Pathway

Figure 2

Cell viability assay on cancer cells. (a) CAL-62, MDA-MB-231, HeLa, U118, HepG2, and A549 cells treated with CAP for 10 s, 20 s, 30 s, and 40 s, or without CAP treatment. (b) The effect of NAC on the viability of CAL-62 cells. (c) CAL-62 cells treated with the gas of helium, argon, and air for 15 s. (d) CAL-62 cells treated with CAP for 0 s, 5 s, 10 s, 15 s, 20 s, and 30 s, and CAP treated in the different oxygen ratios of working gases. At 24 hours after plasma treatment, cell viability was measured by the MTT assay. Data represent the mean ± SD of three independent experiments. , , and with ANOVA compared with the control.

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