Genotypic Characterization of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in Southeast Asian Aquaculture

Table 1

Description of samples used to evaluate ISKNV genomic diversity in Southeast Asian aquaculture.

Sample ID	Collection details		Host		Fish type, habitat	Clinical status	MCV qPCR result		Reference
	Country of origin	Year	Species	Common name			Ct (average)	MCV genome copies/mg
1	Indonesia	2011	Trichogaster leeri	Pearl gourami	Ornamental, fresh water	Subclinical	20.38	7.81 × 10^7	[14]
2	Thailand	2011	Trichogaster trichopterus	Blue/gold gourami	Ornamental, fresh water	Subclinical	25.06	2.92 × 10^6	[14]
3	Indonesia	2015	Pterapogon kauderni	Banggai cardinalfish	Ornamental, marine	Subclinical	17.9	4.42 × 10^8	[44]
4	Indonesia	2014	(♀) Epinephelus fuscoguttatus x (♂) E. polyphekadion	Hybrid grouper cantik	Food fish, marine	Subclinical	28.57	2.50 × 10^4	[45]
5	Indonesia	2014	(♀) E. fuscoguttatus x (♂) E. polyphekadion	Hybrid grouper cantik	Food fish, marine	Subclinical	21.84	2.80 × 10^7	[45]
6	Thailand	2015	Trichopodus trichopterus	Three spot gourami	Ornamental, fresh water	Subclinical	14.57	4.58 × 10^9	[44]
7	Malaysia	2015	Xiphophorus helleri	Green swordtail	Ornamental, fresh water	Subclinical	21.16	4.50 × 10^7	[44]
8	Thailand	2015	Trichogaster lalius	Dwarf gourami	Ornamental, fresh water	Subclinical	16.2	1.46 × 10^9	[44]
9	Sri Lanka	2015	Xiphophorus maculatus	Southern platyfish	Ornamental, fresh water	Subclinical	19.22	1.76 × 10^8	[44]
10	Indonesia	2015	(♀) E. fuscoguttatus x (♂) E. polyphekadion	Hybrid grouper	Food fish, marine	Subclinical	24.49	2.72 × 10^7	[15]
11	SE Asia	2010	Trichogaster lalius	Dwarf gourami	Ornamental, fresh water	Clinical	18.52	2.86 × 10^8	[46]
12	Indonesia	2016	(♀) E. fuscoguttatus x (♂) E. lanceolatus	Hybrid grouper cantang	Food fish, marine	Clinical	14.71	4.15 × 10^9	[25]
13	Indonesia	2018	Osphronemus goramy	Giant gourami	Food fish, fresh water	Clinical	24.65	3.91 × 10^6	This study
14	Indonesia	2018	Osphronemus goramy	Giant gourami	Food fish, fresh water	Clinical	16.51	1.17 × 10^9	This study
15	Indonesia	2016	(♀) E. fuscoguttatus x (♂) E. polyphekadion	Hybrid grouper cantik	Food fish, marine	Clinical	19.33	1.63 × 10^8	[47]
16	Indonesia	2014	(♀) E. fuscoguttatus x (♂) E. polyphekadion	Hybrid grouper cantik	Food fish, marine	Subclinical	24.49	4.37 × 10^6	[45]

the isolate was sequenced from the 4^th in vivo passage in Murray cod (Maccullochella peelii) by Fusianto et al. [48].