Research Article

Genotypic Characterization of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in Southeast Asian Aquaculture

Table 5

Amino acid similarity for gene-by-gene comparison within the predicted ORFs in the samples from the present study and reference samples from similar genotypes. Data are the sum of genes within the categories for percentage similarity compared to angelfish iridovirus AFIV-16 (ISKNV genotype, MK689685), pompano iridovirus isolate PIV 2014a (RSIV genotype clade 1, MK098186 PIV 2014), and RSIV isolate KagYT96 (RSIV genotype clade 2, MK689686).

Gene similarity (pairwise amino acid (%))12345678910111213141516

Compared with ISKNV (MK689685 angelfish iridovirus AFIV-16)
1001039530105105939894101104104105921041052
<100–9514207412122419221512121125131156
<95–90231122222222432332
<90–8500000101101000010
<85–800010000100000002
<80–751001100010001015
<75–701011100110100113
<7003400120031101010

Compared with RSIV clade 1 (MK098186 PIV 2014)
10022122222222222221
<100–9556555756565656565656565654555682
<95–903534333535353534353535353736356
<90–857897777877787782
<85–805565555554545542
<80–756656666666666663
<75–701111121112111110
<7091099989999999994

Compared with RSIV clade 2 (MK689686 RSIV isolate KagYT 96)
100221222222222222107
<100–9559596659595859595959595959595910
<95–903029253030303029302930303030301
<90–851414131414141414141414141314140
<85–801111111111112110
<80–754454454543444440
<75–701101121112111110
<707877767778777770