A Universal and Efficient Detection of Chytridiomycosis Infections in Amphibians Using Novel Quantitative PCR Markers

Table 1

Comparison of TaqMan-based qPCR assay using Boyle’s primers and SYBR-green based qPCR using the new primers. Samples that are positive in both the assays were true positive (TP), positive in Boyle’s assay and negative in the new assay as false negative (FN), positive for the new assay and negative for Boyle’s assay as false positive (FP) and negative for both as true negative (TN).

Boyle’s Primer set	New Primer set
		Positive	Negative
	Positive	31	4
	Negative	2	23