Isolation and Genetic Characterization of the Human Relapsing Fever Spirochete Borrelia persica from a Dog with Improved Cultivation Techniques

Table 1

Target genes and primers used for PCR to detect and characterize B. persica, E. canis, Babesia spp., and Hepatozoon spp.

Pathogen	Target gene	Primers	Amplicon size (bp)	Primer sequence (5^′−3^′)	Reference
Borrelia spp.	flaB	Bfpbu	346	GCTGAAGAGCTTGGAATGCAACC	[39]
		Bfpcr		TGATCAGTTATCATTCTAATAGCA
		BOR1	750	TAATACGTCAGCCATAAATGC	[42]
		BOR2		GCTCTTTGATCAGTTATCATTC
	glpQ	glpQ-510f	280	AAAACCCTTTTGGCATAAACAACA	[40]
		glpQ-770r		CCAGGGTCCAATTCCGTCAG
	16S rRNA	REC4	515	ATGCTAGAAACTGCATGA	[41]
		REC9		TCGTCTGAGTCCCCATCT
E. canis	16S rRNA	E.c. 16S-fwd	123	TCGCTATTAGATGAGCCTACGT	[43]
		E.c. 16S-rev		GAGTCTGGACCGTATCTCAG
Babesia spp. and Hepatozoon spp.	18S rRNA	Piroplasmid-F	350	CCAGCAGCCGCGGTAATTC	[44]
		Piroplasmid-R		CTTTCGCAGTAGTTYGTCTTTAACAAATC