Development of Live Vaccine Candidates for Canine Influenza H3N2 Using Naturally Truncated NS1 Gene

Figure 2

Generation of CIV^SA1 LAIV by reverse genetics: NS gene of A/equine/Kyonggi/SA1/2011 (SA1) was introduced into A/canine/Korea/01/2007 to generate CIV^SA1 LAIV by using plasmid-based reverse genetic technology (a). SANGER sequence analysis confirmed that the NS gene of CIV^SA1 was identical with that of SA1 (b). The 23-nt deletion results in a frame shift, thereby, generating in-frame early stop codon (c).

(a)

(b)

(c)