Development of Live Vaccine Candidates for Canine Influenza H3N2 Using Naturally Truncated NS1 Gene

Figure 4

Antiviral gene mRNA levels and viral replication in lung from mice infected with CIV or CIV^SA1. Mice were intranasally inoculated with CIV (n = 12), CIV^SA1 (n = 12), and PBS (n = 12), respectively. The viral infectious dose was 10⁵ EID₅₀/mouse. Euthanization was performed on each three mice to collect lungs on dpi 3, 5, 7, and 9. The mRNA expression levels in those lungs were measured by real-time PCR and analyzed using the Pfaffl method. The GAPDH housekeeping gene was used as an endogenous standard gene (a). Mice were intranasally infected with CIV (n = 9), CIV^SA1 (n = 9), and PBS (n = 5), respectively. The viral infectious dose was 10^6.5 EID₅₀/mouse. The viral titer of lung and nasal wash samples from dpi 4 euthanized mice was measured by TCID₅₀ method (b). The results were expressed as mean ± SD and Statistical significance was calculated using two-tailed Student’s t test (, , ).

(a)

(b)