Development of Live Vaccine Candidates for Canine Influenza H3N2 Using Naturally Truncated NS1 Gene

Figure 6

Analysis of cellular immunity in both spleen and lung of mice inoculated with CIV or CIV^SA1. Mice were inoculated with CIV (n = 12), CIV^SA1 (n = 12), and PBS (n = 12), respectively. The viral infectious dose was 10⁵ EID₅₀/mouse. Euthanize was performed on each three mice to collect lungs on dpi 3, 5, 7, and 9. FACS was performed in spleen to determine the percentage of CD4⁺IFN-γ⁺ cells (a), CD4⁺TNF-α⁺ cells (b), and CD4⁺Foxp3⁺ cells (c). ELISPOT assay for IFN-γ was performed on collected spleens (d) and lungs (e). The results were expressed as the mean ± SD. Statistical significance was calculated using two-tailed Student’s t test (, , ).

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