Development of Live Vaccine Candidates for Canine Influenza H3N2 Using Naturally Truncated NS1 Gene

Figure 7

Determining both humoral and mucosal antibody titers against CIV. ELISA assay was performed to measure the serum IgG (a and e) and BALF IgA (d and h) antibody titers. The O.D. values were measured at 450 nm wavelength. The serum HI (b and f) and SN (c and g) antibody titer were analyzed using CIV whole virus. Serum and BALF samples were collected from mice at 2 weeks after the 1st (a–d) and 2nd (e–h) vaccine. The OD450 values of 1 : 2¹³ dilution of serum IgG antibody samples (a and e) and 1 : 2³ dilution of BALF IgA antibody samples (d and h) were compared among the different groups. The results were expressed as the mean ± SD, and the number of samples for each experiment was indicated in the respective figures. Statistical significance was calculated using a one-way analysis of variance (one-way ANOVA). (, ).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)