Development of Live Vaccine Candidates for Canine Influenza H3N2 Using Naturally Truncated NS1 Gene

<div>Determining cellular immunity against CIV. In the 2nd week after the 2nd vaccination, mice were euthanized to obtain the spleen (<i>n</i> = 5) and lungs (<i>n</i> = 5). The acquired spleen and lung tissues were subsequently separated into single cells. Following treatment with CIV at a dose of 10<sup>5.5</sup> EID/100 <i>μ</i>l for splenocytes (a) and pneumocytes (b), mouse IFN-<i>γ</i> ELISPOT analyses were conducted. The results were expressed as the mean ± SD. 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Transboundary and Emerging Diseases

fig8

Figure 8

Figure 8: Development of Live Vaccine Candidates for Canine Influenza H3N2 Using Naturally Truncated NS1 Gene 