Species, Natural Leishmania spp. Detection and Blood Meal Sources of Phlebotomine Sandflies (Diptera: Psychodidae: Phlebotominae) in Peridomiciles from a Leishmaniases Endemic Area of Brazil

Figure 2

RFLP-PCR products from the Leishmania spp. kDNA digested with Hae III restriction enzyme in captured-females Phlebotomine sandflies DNA samples from Andradina and Ilha Solteira county, São Paulo state by CDC light traps between 2018 and 2020. Footnote: Capillary electrophoresis gel for kDNA Leishmania spp. PCR products after digestion by Hae III. DNA samples of L. (L.) amazonensis reference strain (IFLA/BR/1967/ph8) (L.a), L. (V.) braziliensis reference strain (MCAN/BR/1987/CÃO21) (L.b), L. (L.) infantum reference strain (MCAN/BR/1984/CCC-17.481) (L.i), Ev. (Ald.) carmelinoi female (1), Ev. (Ald.) lenti female (2), Lu. (Lu.) longipalpis female (3–5), and ultrapure water (negative control–NC).