The Scientific World Journal

The Scientific World Journal / 2002 / Article

Peer-Reviewed Protocol | Open Access

Volume 2 |Article ID 103821 | 5 pages | https://doi.org/10.1100/tsw.2002.839

OptiPrepTM Density Gradient Solutions for Nonmammalian Organelles

Received07 Mar 2002
Accepted15 May 2002

Abstract

Any density gradient for the isolation of nonmammalian organelles should ideally only expose the sedimenting biological particles to an increasing concentration of the gradient solute. Thus they will experience only an increasing density and viscosity, other parameters such as osmolality, pH, ionic strength and the concentration of important additives (such as EDTA and DTT) should remain as close to constant as possible. This Protocol Article describes the strategies for the dilution of OptiPrep™ in order to prepare such solutions for organelles and membranes from nonmammalian sources such as yeast.


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