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TheScientificWorldJOURNAL
Volume 2, Pages 1347-1350
http://dx.doi.org/10.1100/tsw.2002.283
Peer-Reviewed Protocol

Fractionation of Hepatic Nonparenchymal Cells

School of Biomolecular Sciences, Liverpool John Moores University, UK

Received 7 March 2002; Revised 27 March 2002

Copyright © 2002 John Graham.

Abstract

The majority of parenchymal cells from mammalian liver cells can be removed by very low speed centrifugation (50 g) but a simple low-density barrier (1.096 g/ml) is required to remove the remaining parenchymal cells from the 50-g supernatant which contains all of the lower density nonparenchymal cells. Continuous gradients of Nycodenz® can provide satisfactory resolution of Kupffer, stellate, and endothelial cells on an analytical basis but the separation of different cell types is not sufficient preparatively. Flotation through a low-density iodixanol barrier can, however, provide a satisfactory enrichment of the least dense nonparenchymal cell – the stellate cells.