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TheScientificWorldJOURNAL
Volume 2, Pages 1634-1637
http://dx.doi.org/10.1100/tsw.2002.850
Peer-Reviewed Protocol

Isolation of Rat and Human Hippocampal Neuron Fractions in a Discontinuous Density Gradient

School of Biomolecular Sciences, Liverpool John Moores University, UK

Received 7 March 2002; Revised 14 May 2002; Accepted 15 May 2002

Copyright © 2002 John Graham.

Abstract

The plating efficiency of neurons in culture is highly dependent on the concentration of cells used to establish the monolayer. A discontinuous iodixanol gradient permits both the production of a viable concentrated suspension of neurons and purification from other brain tissue elements. The gradient that is described in this Protocol Article is applicable to brain tissue from rat and also from human biopsy specimens.