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TheScientificWorldJOURNAL
Volume 2 (2002), Pages 1626-1629
http://dx.doi.org/10.1100/tsw.2002.849
Peer-Reviewed Protocol

Homogenization of Mammalian Tissues

School of Biomolecular Sciences, Liverpool John Moores University, UK

Received 7 March 2002; Revised 14 May 2002; Accepted 15 May 2002

Copyright © 2002 John Graham.

Abstract

Satisfactory homogenization of a tissue is a necessary prerequisite to any fractionation schedule. A detailed protocol is given for rat liver because of the widespread use of this tissue. Although this technique should be broadly applicable to any soft tissue and to any subsequent fractionation procedure, there are certain tissues and applications that require either minor or extensive modification. Some of these points are addressed in the Notes section.