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TheScientificWorldJOURNAL
Volume 2, Pages 1650-1653
http://dx.doi.org/10.1100/tsw.2002.853
Peer-Reviewed Protocol

Purification of Parietal and Chief Cells from the Gastric Mucosa

School of Biomolecular Sciences, Liverpool John Moores University, UK

Received 7 March 2002; Accepted 15 May 2002

Copyright © 2002 John Graham.

Abstract

Acid-secreting parietal cells from the gastric mucosa are widely studied as a model in studies on ion transport. A discontinuous gradient of iodixanol has been found to be superior to earlier protocols using Nycodenz® and this method, which removes a significant amount of contaminating cells and mucus is a very useful prelude to further purification by elutriation.