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Volume 2, Pages 1400-1443
Peer-Reviewed Protocol

Purification of Peroxisomes Using a Density Barrier in a Swinging-Bucket Rotor

School of Biomolecular Sciences, Liverpool John Moores University, UK

Received 7 March 2002; Revised 14 May 2002; Accepted 15 May 2002

Copyright © 2002 John Graham.


In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a preformed isosmotic continuous gradient. Because of the large difference in density between peroxisomes and the next densest organelle (mitochondria), a density barrier is effective. The resolution of the peroxisomes is far superior than that in sucrose and, unlike in Percoll®, there is no contamination from endoplasmic reticulum.