The Scientific World Journal

The Scientific World Journal / 2002 / Article
Special Issue

Analysis, Toxicity and Biodegradation of Organic Pollutants in Groundwater from Contaminated Land, Landfills and Sediments: Selected Peer-Reviewed

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Volume 2 |Article ID 548435 |

Vitalia S. Krikounova, Ramadan Abuknesha, Sergei A. Eremin, "Express Detection of Pentachlorophenol as Dioxins Precursor in Natural Water", The Scientific World Journal, vol. 2, Article ID 548435, 6 pages, 2002.

Express Detection of Pentachlorophenol as Dioxins Precursor in Natural Water

Received12 Nov 2001
Revised06 Mar 2002
Accepted10 Mar 2002


A rapid detection method for the pesticide pentachlorophenol (PCP) — polarization fluoroimmunoassay (PFIA) — in the dynamic range of 10–9,000 ppb was developed. PCP may form polychlorinated dibenzo-p-dioxins, making environmental monitoring of this compound an issue of great importance. In order to optimize the PFIA procedure, a number of fluorescein-labeled PCP derivatives and similar compounds (tracers) were synthesized, and the influence of their structure on PFIA characteristics was studied. Also, two antisera were tested in developing PFIA for PCP. The developed method is highly specific for PCP and can be used for its determination in water samples at a level down to 10 ppb. Total time of the assay for 10 samples is about 7 min. The assay provides a useful and a highly practical screening tool for the processing of large numbers of samples and for the preliminary estimation of potential dioxins contamination in water resources.

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