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Volume 2, Pages 818-826
Research Article

Screening and Selection of Hypoallergenic Buckwheat Species

1Kiyomoto Bio Co. Ltd., 2-28-4 Sakura, Gakuen Kibanadai, Miyazaki 889-2154, Japan
2Lab of Plant Genes and Physiology, College of Agriculture, Osaka Prefecture University, 1-1, Gakuen-Cho, Sakai, Osaka 599-8531, Japan

Received 3 December 2001; Revised 29 January 2002; Accepted 4 February 2002

Copyright © 2002 Arun Nair and Taiji Adachi.


Both common buckwheat (Fagopyrum esculentum) flour and meal cause an allergy in sensitive patients, and if unnoticed, it can be fatal. It has become a potential occupational hazard for some mill workers. The development of hypoallergenic buckwheat would be more efficient if natural mutants for allergenic protein are detected. A screening and selection method was developed using SDS-PAGE coupled with PCR techniques. SDS-PAGE analysis of 14 different species of buckwheat revealed that F. lineare and F. urophyllum lack the 22-kDa major allergenic protein. PCR-based screening with specific primers for sequences encoding the allergenic protein was also effective in distinguishing the allergen-deficient species.