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TheScientificWorldJOURNAL
Volume 2 (2002), Pages 818-826
http://dx.doi.org/10.1100/tsw.2002.157
Research Article

Screening and Selection of Hypoallergenic Buckwheat Species

1Kiyomoto Bio Co. Ltd., 2-28-4 Sakura, Gakuen Kibanadai, Miyazaki 889-2154, Japan
2Lab of Plant Genes and Physiology, College of Agriculture, Osaka Prefecture University, 1-1, Gakuen-Cho, Sakai, Osaka 599-8531, Japan

Received 3 December 2001; Revised 29 January 2002; Accepted 4 February 2002

Copyright © 2002 Arun Nair and Taiji Adachi.

Abstract

Both common buckwheat (Fagopyrum esculentum) flour and meal cause an allergy in sensitive patients, and if unnoticed, it can be fatal. It has become a potential occupational hazard for some mill workers. The development of hypoallergenic buckwheat would be more efficient if natural mutants for allergenic protein are detected. A screening and selection method was developed using SDS-PAGE coupled with PCR techniques. SDS-PAGE analysis of 14 different species of buckwheat revealed that F. lineare and F. urophyllum lack the 22-kDa major allergenic protein. PCR-based screening with specific primers for sequences encoding the allergenic protein was also effective in distinguishing the allergen-deficient species.