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TheScientificWorldJOURNAL
Volume 2, Pages 1838-1842
http://dx.doi.org/10.1100/tsw.2002.855
Short Communication

Semisynthesis of Ribonuclease A using Intein-Mediated Protein Ligation

1Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA
2Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA
3Martin-Luther University Halle-Wittenberg, Department of Biochemistry and Biotechnology, Kurt-Mothes Strasse 3, 06120 Halle, Germany

Received 19 March 2002; Revised 15 May 2002; Accepted 16 May 2002

Copyright © 2002 Ulrich Arnold et al.

Citations to this Article [10 citations]

The following is the list of published articles that have cited the current article.

  • Sm Fuchs, and Rt Raines, “Polyarginine as a multifunctional fusion tag,” Protein Science, vol. 14, no. 6, pp. 1538–1544, 2005. View at Publisher · View at Google Scholar
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  • Ulrich Arnold, “Incorporation of non-natural modules into proteins: structural features beyond the genetic code,” Biotechnology Letters, vol. 31, no. 8, pp. 1129–1139, 2009. View at Publisher · View at Google Scholar
  • Christian Piontek, Petra Ring, Olaf Harjes, Christian Heinlein, Stefano Mezzato, Nelson Lombana, Claudia Pöhner, Markus Püttner, Daniel Varón Silva, Andreas Martin, Franz Xaver Schmid, and Carlo Unverzagt, “Semisynthese eines homogenen Glycoprotein-Enzyms: Ribonuclease C (Teil 1),” Angewandte Chemie, vol. 121, no. 11, pp. 1968–1973, 2009. View at Publisher · View at Google Scholar
  • Christian Piontek, Petra Ring, Olaf Harjes, Christian Heinlein, Stefano Mezzato, Nelson Lombana, Claudia Poehner, Markus Puettner, Daniel Varon Silva, Andreas Martin, Franz Xaver Schmid, and Carlo Unverzagt, “Semisynthesis of a Homogeneous Glycoprotein Enzyme: Ribonuclease C: Part 1,” Angewandte Chemie-International Edition, vol. 48, no. 11, pp. 1936–1940, 2009. View at Publisher · View at Google Scholar
  • Nicholas A. McGrath, and Ronald T. Raines, “Chemoselectivity in Chemical Biology: Acyl Transfer Reactions with Sulfur and Selenium,” Accounts Of Chemical Research, vol. 44, no. 9, pp. 752–761, 2011. View at Publisher · View at Google Scholar
  • Ulrich Arnold, Bayard R. Huck, and Samuel H. Gellman, “Protein prosthesis: beta-peptides as reverse-turn surrogates,” Protein Science, vol. 22, no. 3, pp. 274–279, 2013. View at Publisher · View at Google Scholar
  • Ulrich Arnold, and Ronald T. Raines, “Replacing a single atom accelerates the folding of a protein and increases its thermostability,” Org. Biomol. Chem., 2016. View at Publisher · View at Google Scholar