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Volume 2, Pages 1607-1609
Peer-Reviewed Protocol

Isolation of Human Platelets (Thrombocytes)

School of Biomolecular Sciences, Liverpool John Moores University, UK

Received 7 March 2002; Accepted 6 May 2002

Copyright © 2002 John Graham.


Platelets from human blood can be isolated in high yield by centrifugation of whole blood over an iodixanol density barrier of 1.063 g/ml. The separation from all of the blood cells (which form a pellet) is based on the slower sedimentation velocity of the smaller platelets.