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TheScientificWorldJOURNAL
Volume 2, Pages 1526-1539
http://dx.doi.org/10.1100/tsw.2002.838
Peer-Reviewed Protocol

Purification of Peroxisomes in a Preformed Iodixanol Gradient in a Fixed-Angle Rotor

School of Biomolecular Sciences, Liverpool John Moores University, UK

Received 7 March 2002; Revised 14 May 2002; Accepted 15 May 2002

Copyright © 2002 John Graham.

Abstract

In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a preformed isosmotic continuous gradient. The resolution of the peroxisomes is far superior than that in sucrose and, unlike in Percoll® there is no contamination from endoplasmic reticulum.