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TheScientificWorldJOURNAL
Volume 2, Pages 1209-1215
http://dx.doi.org/10.1100/tsw.2002.196
Mini-Review Article

The ‘Relevant’ Stability of Proteins with Equilibrium Intermediates

1Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, 50009-Zaragoza, Spain
2The Scripps Research Institute, Molecular Biology TPC-28, 10550 North Torrey Pines Road, La Jolla, CA 9203, USA
3Operón S.A., Camino del Plano 19, Cuarte de Huerva, 50410-Zaragoza, Spain

Received 17 December 2001; Accepted 5 March 2002

Copyright © 2002 Javier Sancho et al.

Citations to this Article [13 citations]

The following is the list of published articles that have cited the current article.

  • Idolka Pedroso, María P. Irún, Claudia Machicado, and Javier Sancho, “Four-state equilibrium unfolding of an scFv antibody fragment,” Biochemistry, vol. 41, no. 31, pp. 9873–9884, 2002. View at Publisher · View at Google Scholar
  • Luis A. Campos, Marta Bueno, Jon Lopez-Llano, María Ángeles Jiménez, and Javier Sancho, “Structure of Stable Protein Folding Intermediates by Equilibrium ϕ-Analysis: The Apoflavodoxin Thermal Intermediate,” Journal of Molecular Biology, vol. 344, no. 1, pp. 239–255, 2004. View at Publisher · View at Google Scholar
  • Luis A. Campos, Maria M. Garcia-Mira, Raquel Godoy-Ruiz, Jose M. Sanchez-Ruiz, and Javier Sancho, “Do Proteins Always Benefit from a Stability Increase? Relevant and Residual Stabilisation in a Three-state Protein by Charge Optimisation,” Journal of Molecular Biology, vol. 344, no. 1, pp. 223–237, 2004. View at Publisher · View at Google Scholar
  • Marta Bueno, Luis A. Campos, Jorge Estrada, and Javier Sancho, “Energetics of aliphatic deletions in protein cores,” Protein Science, vol. 15, no. 8, pp. 1858–1872, 2006. View at Publisher · View at Google Scholar
  • Marta Bueno, Nunilo Cremades, José Luis Neira, and Javier Sancho, “Filling Small, Empty Protein Cavities: Structural and Energetic Consequences,” Journal of Molecular Biology, vol. 358, no. 3, pp. 701–712, 2006. View at Publisher · View at Google Scholar
  • L.A. Campos, and J. Sancho, “Native-specific stabilization of flavodoxin by the FMN cofactor: Structural and thermodynamical explanation,” Proteins: Structure, Function and Genetics, vol. 63, no. 3, pp. 581–594, 2006. View at Publisher · View at Google Scholar
  • J. Sancho, “Flavodoxins: Sequence, folding, binding, function and beyond,” Cellular and Molecular Life Sciences, vol. 63, no. 7-8, pp. 855–864, 2006. View at Publisher · View at Google Scholar
  • N. Cremades, and J. Sancho, “Molten Globule and Native State Ensemble of Helicobacter pylori Flavodoxin: Can Crowding, Osmolytes or Cofactors Stabilize the Native Conformation Relative to the Molten Globule?,” Biophysical Journal, vol. 95, no. 4, pp. 1913–1927, 2008. View at Publisher · View at Google Scholar
  • María Larriva, Lidia Prieto, Pierpaolo Bruscolini, and Antonio Rey, “A simple simulation model can reproduce the thermodynamic folding intermediate of apoflavodoxin,” Proteins: Structure, Function and Bioinformatics, vol. 78, no. 1, pp. 73–82, 2010. View at Publisher · View at Google Scholar
  • Janina Speck, Jochen Hecky, Heng-Keat Tam, Katja M. Arndt, Oliver Einsle, and Kristian M. Müller, “Exploring the Molecular Linkage of Protein Stability Traits for Enzyme Optimization by Iterative Truncation and Evolution,” Biochemistry, vol. 51, no. 24, pp. 4850–4867, 2012. View at Publisher · View at Google Scholar
  • Javier Sancho, “The stability of 2-state, 3-state and more-state proteins from simple spectroscopic techniques... plus the structure of the equilibrium intermediates at the same time,” Archives of Biochemistry and Biophysics, vol. 531, no. 1-2, pp. 4–13, 2013. View at Publisher · View at Google Scholar
  • Emilio Lamazares, Isabel Clemente, Marta Bueno, Adrián Velázquez-Campoy, and Javier Sancho, “Rational stabilization of complex proteins: a divide and combine approach,” Scientific Reports, vol. 5, pp. 9129, 2015. View at Publisher · View at Google Scholar
  • Nathan W Gardner, Sarah M McGinness, Jainik Panchal, Elizabeth M. Topp, and Chiwook Park, “A cooperative folding unit as the structural link for energetic coupling within a protein,” Biochemistry, 2017. View at Publisher · View at Google Scholar