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TheScientificWorldJOURNAL
Volume 3, Pages 342-347
http://dx.doi.org/10.1100/tsw.2003.30
Peer-Reviewed Protocol

Molluscan Shell Matrix Characterization by Preparative SDS-PAGE

IsoTis, NV., ProfBronkhorstlaan, 10, Gebouw D, 3723 MB Bilthoven, The Netherlands

Copyright © 2003 Frederic�Marin.

Abstract

The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro characterization. After dissolution of the mineral phase, the glycoproteins are fractionated �blind� on a preparative electrophoresis. They are subsequently detected with a polyclonal antibody raised against the whole matrix.