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TheScientificWorldJOURNAL
Volume 3, Pages 51-58
http://dx.doi.org/10.1100/tsw.2003.07
Research Article

Apoptosis Gene Hunting Using Retroviral Expression Cloning: Identification of Vacuolar ATPase Subunit E

School of Life Sciences, Keele University, Keele, Staffordshire, ST5 5BG, England

Academic Editor: Leonid A. Gavrilov

Copyright © 2003 Claire L. Anderson and Gwyn T. Williams.

Abstract

Over the past 10-15 years there has been an explosion of interest in apoptosis. The delayed realisation that cell death is an essential part of life for any multicellular organism has meant that, despite the recent and rapid developments of the last decade, the precise biochemical pathways involved in apoptosis remain incomplete and potentially novel genes may, as yet, remain undiscovered. The hunt is therefore on to bridge the remaining gaps in our knowledge. Our contribution to this research effort utilises a functional cloning approach to isolate important regulatory genes involved in apoptosis. This mini-review focuses on the use and advantages of a retroviral expression cloning strategy and describes the isolation and identification of one such potential apoptosis regulatory gene, namely that encoding vacuolar ATPase subunit E.