Research Article | Open Access
Tamar Kartvelishvili, Marina Abuladze, Nino Asatiani, Joseph Akhvlediani, Eugene Kiziria, Lali Asanishvili, Lia Lejava, Hoi-Ying N. Holman, Nelly Sapojnikova, "Estimation of the Cellular Antioxidant Response to Chromium Action Using ESR Method", The Scientific World Journal, vol. 4, Article ID 249428, 10 pages, 2004. https://doi.org/10.1100/tsw.2004.136
Estimation of the Cellular Antioxidant Response to Chromium Action Using ESR Method
In the present study, the antioxidant capacity of chromium-treated L-41 (human epithelial-like cells) was investigated by the ESR spin-trapping technique. The crude cell extracts of the cells grown in the presence of 2 µM (nontoxic) and 20 µM (toxic) chromium (VI) concentrations were tested in the model Fenton system with and without catalase-inhibitor sodium azide. The presented approach using the ESR technique along with inhibitors lets us discern cell extract defense capacity connected with the enzymatic activity in viable cells and the catabolic activity in dying cells.