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Volume 6 (2006), Pages 20-29
Research Article

Electrochemical Study of Biotin-Modified Self-Assembled Monolayers: Recommendations for Robust Preparation

1Analytical Science Group, National Physical Laboratory, Teddington, Middlesex, TW11 0LW, UK
2Department of Chemical Engineering and Chemical Technology, Imperial College, London, SW7 2AY, USA

Received 11 November 2005; Revised 19 December 2005; Accepted 17 January 2006

Copyright © 2006 Richard J.C. Brown and Dan J.L. Brett.


The development of the underpinning methodology for the production of robust, well-formed, and densely packed biotin-HPDP functionalised gold surfaces, the crucial first step in immobilising bimolecules on surfaces, is described. Self-assembled monolayers (SAMs) with biotin end-groups were prepared on polycrystalline gold surfaces according to a published method. The layers formed were studied using cyclic voltammetry to determine the composition of the layer and its quality. Crystal impedance spectroscopy was also applied as a complimentary indicator of the composition of the layer.For the first time, the effect of assembly time on the properties of the layer was studied along with the composition of the layer and the ability of the precursor molecule to self-assemble by oxidative addition.