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TheScientificWorldJOURNAL
Volume 6, Pages 1609-1618
http://dx.doi.org/10.1100/tsw.2006.269
Mini-Review Article

Integrated Confocal and Scanning Probe Microscopy for Biomedical Research

Rayne Institute, Department of Medicine, University College London, London WC1E 6JJ, UK

Received 24 October 2006; Accepted 22 November 2006

Academic Editor: Margaret Tzaphlidou

Copyright © 2006 B.J. Haupt et al.

Abstract

Atomic force microscopy (AFM) continues to be developed, not only in design, but also in application. The new focus of using AFM is changing from pure material to biomedical studies. More frequently, it is being used in combination with other optical imaging methods, such as confocal laser scanning microscopy (CLSM) and fluorescent imaging, to provide a more comprehensive understanding of biological systems. To date, AFM has been used increasingly as a precise micromanipulator, probing and altering the mechanobiological characteristics of living cells and tissues, in order to examine specific, receptor-ligand interactions, material properties, and cell behavior. In this review, we discuss the development of this new hybrid AFM, current research, and potential applications in diagnosis and the detection of disease.