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TheScientificWorldJOURNAL
Volume 8 (2008), Pages 1088-1097
http://dx.doi.org/10.1100/tsw.2008.136
Research Article

Detection of Heteromers Formed by Cannabinoid CB1, Dopamine D2, and Adenosine A2A G-Protein-Coupled Receptors by Combining Bimolecular Fluorescence Complementation and Bioluminescence Energy Transfer

1Institut d'Investigacions Biomèques August Pi i Sunyer Centro de Investigación Biomédica en Red Sobre Enfermedades Neurodegenerativas (CIBERNED), y Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universitat de Barcelona, Spain
2Unitat de Farmacologia, Departamento Patologia i Terapèica Experimental, Facultat de Medicina, Universitat de Barcelona, L'Hospitalet de Llobregat, Spain
3CIMA Neurociencias, Universidad de Navarra, Pamplona, Spain

Received 23 July 2008; Revised 1 October 2008; Accepted 1 October 2008

Academic Editor: Sergi Ferre

Copyright © 2008 Gemma Navarro et al.

Abstract

Functional interactions in signaling occur between dopamine D2 (D2R) and cannabinoid CB1 (CB1R) receptors, between CB1R and adenosine A2A (A2AR) receptors, and between D2R and A2AR. Furthermore, direct molecular interactions have been reported for the pairs CB1R-D2R, A2AR-D2R, and CB1R-A2AR. Here a combination of bimolecular fluorescence complementation and bioluminescence energy transfer techniques was used to identify the occurrence of D2R-CB1R-A2AR hetero-oligomers in living cells.