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TheScientificWorldJOURNAL
Volume 8 (2008), Pages 346-349
http://dx.doi.org/10.1100/tsw.2008.51
Short Communication

A Tentative Mechanism of Solubilization of Neuropathy Target Esterase from Chicken Embryo Brain by Phospholipase A2

Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI 96822, USA

Received 28 December 2007; Accepted 5 March 2008

Academic Editor: John A. Thomas

Copyright © 2008 Josef┬áSeifert.

Abstract

The neuropathy target esterase is a membrane-bound enzyme linked to organophosphate-induced distal neuropathy. Here we report a tentative mechanism of its solubilization from chicken embryo brains by using phospholipase A2. The enzyme was released from brain membranes after degradation of their structural phospholipids initiated by phospholipase A2. L-α-lysophosphatidylcholine, tested as a representative product of phospholipid hydrolysis, was identified as a new efficient detergent for solubilization of the neuropathy target esterase.