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Volume 10, Pages 2238-2247
Research Article

Correlative Microscopy of Bone in Implant Osteointegration Studies

1Department of Human Anatomical Sciences and Physiopathology of the Locomotor Apparatus (S.A.U. e F.A.L.), Bologna University, Italy
2Department of Human Morphology, Insubria University, Varese, Italy

Received 17 September 2010; Revised 3 November 2010; Accepted 5 November 2011

Academic Editor: Margaret Tzaphlidou

Copyright © 2010 Alessandra Triré al.


Routine morphological analyses usually include investigations by light microscopy (LM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Each of these techniques provides specific information on tissue morphology and all the obtained results are then combined to give an in-depth morphological overview of the examined sample. The limitations of this traditional comparative microscopy lie in the fact that each technique requires a different experimental sample, so that many specimens are necessary and the combined results come from different samples. The present study describes a technical procedure of correlative microscopy, which allows us to examine the same bone section first by LM and then, after appropriate processing, by SEM or TEM. Thanks to the possibility of analyzing the same undecalcified bone sections both by LM and SEM, the approach described in the present study allows us to make very accurate evaluations of old/new bone morphology at the bone-implant interface.