New Role for L-Arginine in Regulation of Inducible Nitric-Oxide-Synthase-Derived Superoxide Anion Production in Raw 264.7 Macrophages
Figure 6
Effect of BH4 on iNOS protein expression, nitrite accumulation, and production in RAW 264.7 cells. Cells were incubated with one of the essential NOS cofactors, BH4 in the presence of DMEM media containing 400 μM of L-arginine. iNOS protein expression in cell lysates, accumulation of nitrite (a), and production in cell supernatants (b) were determined after 24 h of cell incubation with LPS (50 ng/mL). Results represent means ± SEM (). .