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TheScientificWorldJOURNAL
Volume 11, Pages 2613-2619
http://dx.doi.org/10.1100/2011/485381
Clinical Study

Enteropathogenic Escherichia coli Associated with Diarrhea in Children in Cairo, Egypt

1Department of Clinical Pathology, Kasr El Aini Hospital, Cairo University, 12613 Giza, Egypt
2Botany and Microbiology Department, Faculty of Science, Helwan University, 11916 Helwan, Egypt
3Biology Department, Faculty of Science, Jazan University, 2097 Jazan, Saudi Arabia

Received 11 October 2011; Accepted 15 November 2011

Academic Editors: E. L. Jarroll and A. Ouwehand

Copyright © 2011 Iman K. Behiry et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In this study we isolate and identify the Enteropathogenic Escherichia coli (EPEC) causing diarrhea in children less than five years in Cairo, Egypt, during different seasons. Children younger than five years with diarrhea, attending the Pediatric Gastroenterology Intensive Care Unit of the Cairo University Pediatric Hospital in one year period were our group of study. Our control group was age and sex matched concurrent healthy children. The identified E. coli isolates were subjected to antimicrobial disc diffusion susceptibility test and further identified for EPEC serotype by slide agglutination test, using antiserum E. coli somatic trivalent I (O111, O55, O26) according to the instructions of the manufacturer. Out of 134 patients 5.2% of them revealed EPEC in the fecal sample, while the 20 children control group showed no EPEC isolates in their samples. Our EPEC frequency showed variations from the compared results of other studies. Higher rate of EPEC (18.7%) was found in patients between 2 to 3 years, while EPEC rate was (7.5%) in patients less than 6 months old, with 𝑃 < 0 . 0 5 . EPEC was identified from fecal specimens as a unique pathogen or associated with other pathogens in acute and chronic diarrhea in children. EPEC were detected in all seasons except in winter, and was predominant in summer season. Four (57%) EPEC isolates were resistant to ampicillin, ticarcillin, and cotrimoxazole, and (14.3%) to the third generation cephalosporins.