Embryonic Regulation of the Mouse Hematopoietic Niche
Figure 3
Hematopoietic environment in the AGM region. (a) (upper) Model of HC clusters, which are attached to the endothelial wall mainly on the ventral side. (lower) Confocal images of an HC cluster expressing CD34/c-Kit in the aortic region at 10.5 dpc. AGM region tissue sections were prepared from ICR mouse embryos at 10.5 dpc, stained with antibodies, and observed under confocal microscopy. CD34 (red), c-Kit (green), and TOTO-3 (blue). (b) Model of intrinsic and extrinsic regulation at the AGM region. (upper) Transcription factors (such as Runx1) maintain HSC development as intrinsic regulators. Signal transducers (such as Ptch and Smo) and transcription factor (such as Gli1, 2, 3) in mesenchymal cells maintain HSC number and development. (lower) Cytokines (SCF and IL-1) maintain HC clusters and HSC development as extrinsic regulators.
