Embryonic Regulation of the Mouse Hematopoietic Niche
Figure 4
Hematopoietic environment in the placenta. (a) (upper) Model of HC clusters in the placenta. HC clusters are attached to the endothelial wall of capillary vessels, the so-called vascular labyrinth region, from 10.5 dpc to 12.5 dpc. (lower) Confocal images of an HC cluster expressing CD31/c-Kit in the placenta at 10.5 dpc. Sections of placenta were prepared from ICR mouse embryos at 10.5 dpc, stained with indicated antibodies and observed under confocal microscopy. CD31 (red), Kit (green), and TOTO-3 (blue). (b) Model of intrinsic and extrinsic regulation in the placenta. (upper) Transcription factors (such as Runx1, Evi-1, c-Myb) maintain HSC development intrinsically. (lower) Cytokines (such as SCF and IL-3) maintain HSCs as extrinsic regulators.
